Berkeley Lab's Molecular Biophysics & Integrated Bioimaging Division has an opening for a Sr. Research Associate. You will perform, test and optimize procedures for the purification and biophysical characterization of protein complexes in support of our investigations focused on cell membrane-based systems facilitating cellular response to the environment and particularly those associated with the formation of microbial communities. In addition, you will help oversee day-to-day laboratory operations, such as the procurement and maintenance of equipment and supplies.
What You Will Do:
Conduct experiments related to the purification, and occasional expression, of membrane proteins.
Characterize the oligomeric state of membrane protein complexes using native and 2D electrophoresis methods.
Reconstitution of detergent-solubilized membrane proteins into liposomes for subsequent functional characterization studies; perform membrane permeability assays.
Isolate bacterial vesicles and other non-cellular components from liter-scale cultures; characterize the molecular composition and putative function of these samples.
Prepare protein and other biological samples for targeted structural studies (e.g. electron microscopy).
Purify and prepare membrane proteins in forms suitable for metal composition analysis.
Prepare protein samples for mass spectrometry analysis including the development and application of cell shaving techniques.
Characterization and analysis of post-translational modifications, such as lipidation and glycosylation, in selected membrane proteins.
Perform, optimize, and provide input for the development of new biochemical protocols.
Design experiments and achieve milestones in a research environment.
Oversee the ordering of supplies, the repair of equipment, compliance with EH&S policies and procedures and other laboratory maintenance issues.
Accurately prepare solutions, media, and other reagents.
Collaborate with other laboratory members and with researchers of outside institutions.
Assist with organizing and analyzing research results for publication, and presentations.
Additional Responsibilities as needed:
Provide guidance to other members of a team or project.
Provide training to students and lab interns.
Processing and analysis of image data.
What Is Required:
Bachelor's degree, or equivalent experience, and scientific background in protein biochemistry and molecular biology or related field preferred and two to five years experience with increasing responsibility or an M.S. degree.
Research experience (three or more years) in membrane protein biochemistry, purification, and biochemical assays, including:
Detergent solubilization strategies
Membrane protein reconstitution methods
Standard and specialized chromatography media
Preparation of samples for mass spectroscopy and electron microscopy studies
Blue native and 2D PAGE methods
Specialized blotting methods - particularly for lectin-based and affinity labeled targets
Large-scale bacterial cell culture and processing
Experience with automated biochemistry platforms (particularly liquid chromatography systems) used for high-throughput molecular biology protocols and protein purification; also with instrumentation such as spectrophotometers, multi-wavelength media scanners, and plate readers.
Demonstrated ability to interact well with members of a multi-disciplinary research team, have strong written and oral communication, and organizational skills.
The ability to maintain an accurate laboratory notebook.
Experience in using Mac and PC computers, and associated software packages, such as Word, and Excel. Ability to learn custom software applications
Additional Desired Qualifications:
Experience in general molecular biological techniques, especially in the preparation of vectors for membrane protein expression.
Experience in the use of Linux-based computer systems.
The posting shall remain open until the position is filled.
This is a full-time career appointment.
Part-time, 20 hours per week, non-exempt (hourly paid) from overtime.
This position is represented by a union for collective bargaining purposes.
Salary will be determined based on the range by the collective bargaining agreement.
This position may be subject to a background check. Any convictions will be evaluated to determine if they directly relate to the responsibilities and requirements of the position. Having a conviction history will not automatically disqualify an applicant from being considered for employment.
Work will be primarily performed at University of California, Berkeley - Donner Lab, Berkeley 94720-4767
Berkeley Lab (LBNL) addresses the world's most urgent scientific challenges by advancing sustainable energy, protecting human health, creating new materials, and revealing the origin and fate of the universe. Founded in 1931, Berkeley Lab's scientific expertise has been recognized with 13 Nobel prizes. The University of California manages Berkeley Lab for the U.S. Department of Energy's Office of Science.
Equal Employment Opportunity: Berkeley Lab is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status. Berkeley Lab is in compliance with thePay Transparency Nondiscrimination Provisionunder 41 CFR 60-1.4. Clickhere to view the poster and supplement: "Equal Employment Opportunity is the Law."
Internal Number: 88381
About Lawrence Berkeley National Laboratory
In the world of science, Lawrence Berkeley National Laboratory (Berkeley Lab) is synonymous with excellence. Thirteen scientists associated with Berkeley Lab have won the Nobel Prize. Fifty-seven Lab scientists are members of the National Academy of Sciences (NAS), one of the highest honors for a scientist in the United States. Thirteen of our scientists have won the National Medal of Science, our nation's highest award for lifetime achievement in fields of scientific research. Eighteen of our engineers have been elected to the National Academy of Engineering, and three of our scientists have been elected into the Institute of Medicine. In addition, Berkeley Lab has trained thousands of university science and engineering students who are advancing technological innovations across the nation and around the world. Berkeley Lab is a member of the national laboratory system supported by the U.S. Department of Energy through its Office of Science. It is managed by the University of California (UC) and is charged with conducting unclassified research across a wide range of scientific disciplines. Located on a 200-acre site in the hills above the UC Berkeley campus that offers spectacular... views of the San Francisco Bay, Berkeley Lab employs approximately 4,200 scientists, engineers, support staff and students. Its budget for 2011 is $735 million, with an additional $101 million in funding from the American Recovery and Reinvestment Act, for a total of $836 million. A recent study estimates the Laboratory's overall economic impact through direct, indirect and induced spending on the nine counties that make up the San Francisco Bay Area to be nearly $700 million annually. The Lab was also responsible for creating 5,600 jobs locally and 12,000 nationally. The overall economic impact on the national economy is estimated at $1.6 billion a year. Technologies developed at Berkeley Lab have generated billions of dollars in revenues, and thousands of jobs. Savings as a result of Berkeley Lab developments in lighting and windows, and other energy-efficient technologies, have also been in the billions of dollars. Berkeley Lab was founded in 1931 by Ernest Orlando Lawrence, a UC Berkeley physicist who won the 1939 Nobel Prize in physics for his invention of the cyclotron, a circular particle accelerator that opened the door to high-energy physics. It was Lawrence's belief that scientific research is best done through teams of individuals with different fields of expertise, working together. His teamwork concept is a Berkeley Lab legacy that continues today.