The Staff Scientist is part of the Vanderbilt Center for Stem Cell Biology (VCSCB) at Vanderbilt University. This individual is a key team member in both the VCSCB and the Vanderbilt Genome Editing Resource (VGER), an institutional shared resource, and is responsible for the development and performance of services by VGER that are related to the derivation and gene editing of human pluripotent stem cell lines. This position plays an important role at Vanderbilt by enabling investigators to perform state-of-the-art cellular, molecular and/or developmental biology experimentation in pluripotent stem cells. Reporting directly to the Director of the VCSCB, the Staff Scientist will be a vital part of a very capable, motivated and responsible team.
The Staff Scientist will develop new pluripotent stem cell lines, perform genome editing in both cell lines and mice, assist investigators with the design of new genome editing projects, and write both project summaries and parts of scientific manuscripts. The Staff Scientist will strive to improve the efficiencies and experimental sophistication of genome editing technologies provided by the VGER. In addition, to using CRISPR/Cas to perform targeted genetic modifications, the Staff Scientist will also utilize site-specific recombinases, transposases and other genome manipulating technologies. By successfully helping investigators to produce and genetically-modify human pluripotent stem cell lines, the Staff Scientist's performance is critical to the missions of Vanderbilt, the VCSCB, and VGER. A high level of communication, teamwork, organization, and performance is required for success.
The Vanderbilt Center for Stem Cell Biology (VCSCB) reports to the Dean of the Basic Sciences in the School of Medicine. This unit facilitates scientific communication between member faculty and oversees the operations of two fee-for-service based core facilities, the Vanderbilt Genome Editing Resource (VGER) and Creative Data Solutions (CDS). VGER currently supports over one hundred laboratories at Vanderbilt in the production and use of genetically altered mice, and seeks to expand its already substantial scientific impact through the development and performance of services related to the production and genetic modification of human pluripotent stem cell lines.
Duties and responsibilities
Bring a team-oriented approach to the development of new methods and protocols
Perform CRISPR/Cas-mediated mutagenesis in cell lines and mice
Work in cooperation with others to design and perform genome editing experiments
Summarize and present experimental results in a clear, organized and coherent manner
Work in cooperation with others to derive and validate new human pluripotent stem cell lines
Profile of an Ideal Candidate
PhD in the life sciences is necessary.
At least three years of cell culture experience, preferably with human embryonic or induced pluripotent stem cells is necessary.
Knowledge of mammalian development, gene regulation, cell differentiation and genome editing is necessary.
Experience performing CRISPR/Cas-mutagenesis, RNA expression analysis, and induced pluripotent stem cell derivation is preferred.
Basic knowledge of mouse genetics and husbandry is necessary.
Molecular biology skills including tissue culture, PCR, generation and purification of DNA is necessary.
Experience in Microsoft Office including Word, Excel, PowerPoint is necessary.
Experience in the use of FMPro databases and other online data resources is preferred.
About Vanderbilt Benefits
Vanderbilt University offers a competitive, flexible benefits package including health, dental, vision, life, accidental death & dismemberment, disability insurance, paid time off, and a 403(b) retirement plan with employer match. Vanderbilt offers tuition assistance to employees, spouses, and dependent children.
About Vanderbilt University
Vanderbilt University, located in Nashville, Tennessee, is a top-15 private research university offering a full range of undergraduate, graduate and professional degrees. Vanderbilt is situated on a 330-acre campus near the thriving city center, serving more than 12,000 students and employing almost 7,000 faculty and staff. The university's students, staff, and faculty frequently cite Nashville and the surrounding area as one of the many perks of being a part of the Vanderbilt community. Vanderbilt University is a place where your expertise will be valued, your knowledge expanded, and your abilities challenged. It is a place where your diversity is sought and celebrated. Vanderbilt was recently named as one of "America's Best Large Employers" and the top employer in Tennessee and the Nashville metropolitan area in 2019 (Forbes).
Commitment to Equity, Diversity and Inclusion
Vanderbilt University is committed to achieving the goal of a diverse and inclusive academic community of faculty, staff, and students. We seek individuals who are committed to this goal and our campus values.
Internal Number: 1901639
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.