Research - Laboratory/Non-Laboratory, Staff/Administrative
This is a UC San Diego internal recruitment open to UCSD and UCSD Health System staff only.
UCSD Layoff from Career Appointment: Apply by 08/15/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 08/19/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, perform at full operational skill level, conduct experiments in cell signaling and characterize interactions involving growth factors and other proteins in the fields of cancer and neuronal biology. Independently perform experiments making technical determinations and/or observations related to research projects.
Independently perform PCR, cell-based assays, cell culture, protein blotting, and flow cytometry. Conduct limited variety of non-standard procedures. Conduct animal experiments with both mice and rats including histology, staining, and immunohistochemistry. Data processing and presentation and statistical analysis. Contribute to lab administration, compliance, and maintenance.
Theoretical knowledge as required in a Bachelor's degree in biomedical or a related science with two or more years of experience.
Experience and ability to initiate and maintain multiple different eukaryotic cell lines including freezing, thawing, storage, and plating cells in preparation for experiments.
Demonstrated experience with sterile techniques, troubleshooting of contamination problems, and care of incubators and liquid nitrogen freezers.
Theoretical knowledge of molecular biology techniques and genetics and experience in extracting DNA/RNA, PCR, PT-PCR, qPCR.
Proven experience in protein chemistry/purification, gel electrophoresis and transfers, various gel and membrane stains, western blots, and densitometry and analysis on resulting data.
Demonstrated experience with immunohistochemistry.
Demonstrated histology processing, embedding, sectioning, and staining.
Proven experience with mouse and rat handling, surgeries, anesthesia, injections, euthanasia, and tissue harvest.
Knowledge of proper formats, grammar, and spelling in English and proofreading. Ability sufficient to compose, edit, and publish professional correspondence and effectively and accurately document research procedures. Ability to read, comprehend, discuss, and present research materials.
Strong experience with the operation of a personal computer and software applications, such as Microsoft Office (Word, Excel, Access), Photoshop, Acrobat.
Experience with gel/blot imaging and microscopic imaging techniques for both fluorescence and bright field applications and with digital histology image software assisted image processing and analysis.
Knowledge of bio, chemical, and radiation safety techniques and practices. Ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Strong organizational skills to order and maintain lab reagents and supplies, and keep accurate lab records and computer files. Experience in ordering, maintaining, troubleshooting, and calibrating general laboratory equipment (e.g. centrifuges, incubators, microscopes, water baths, etc).
Recent, directly related work experience.
Experience with UCSD ordering, EH&S, and facilities policies and business systems.
Employment is subject to a criminal background check.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.