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The person in this position will work as a lab technician on studies dissecting mechanisms of insulin action that regulate glucose uptake by fat and muscle cells, and defining defects contributing to insulin resistance associated with obesity and type 2 diabetes. Specifically, we focus on the mechanism of cholesterol accumulation in fat and muscle that disrupts cytoskeletal structure essential for glucose transporter GLUT4 regulation by insulin. The primary role will be assisting with ongoing studies asking novel questions about cellular cholesterol accumulation and GLUT4 dysregulation, what mechanisms are involved, and the impact of targeting this toxicity on preventing and/or improving insulin resistance.
Primary Duties and Responsibilities Include:
Taking care of mouse colonies, keeping record of the mice, breeding, genotyping, performing injections, and participate in tissue collection from animals.
Performing in vivo testing including glucose and insulin tolerance tests, subcellular fractionation, lipid assays, confocal microscopy, western blotting, PCR, and ELISA.
Making buffers and reagents for running assays for animal and cell culture studies.
Cell culture including thawing, expanding and freezing cell lines, drug treatment, microscopy and obtaining protein and RNA samples, DNA plasmid isolation and purification.
Transfections and transduction of genes into mammalian cells.
Bachelor’s degree in science from an accredited institution or advanced level science and two years of research experience.
Ability to effectively communicate and exchange information with a wide variety of individuals, excellent interpersonal skills, and a customer service orientation. Strong organizational and multitasking skills including the ability to manage multiple priorities.
Strong background in biological sciences, biochemistry, or molecular biology. Some lab experience or experience with experimental mice.
Working Conditions/Physical Demands:
Ability to perform the essential functions of the position with or without accommodation. Must not have severe allergies to rodents.
Founded in 1820, Indiana University is the state’s public university system, comprised of two core campuses—IU-Bloomington and IUPUI—and six regional campuses located throughout the state. Combined, the total student population is more than 114,000 undergraduate, graduate and professional school students. IU is one of the largest employers in the state, with over 20,000 faculty and staff. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. Diversity and inclusion remains a core commitment to IU’s mission to cultivate an educational environment that supports equal access, participation and representation on all of its campuses and that provides educational and career opportunities for all.