Research - Laboratory/Non-Laboratory, Staff/Administrative
Rutgers, The State University of New Jersey, is seeking a Laboratory Researcher III for ORED. This position works with the Director of the Genome Editing Shared Resource to provide custom gene edited cell lines (mouse, human, other) to investigators of all divisions of Rutgers University, as well as other academic centers. The Lab Researcher III assists with and learns how to design CRISPR gene editing projects, maintains diverse cell lines, establishes electroporation conditions for cell lines, prepares cells for sorting, expands sorted clones, identifies edited cell clones and bank the cell lines. The ability to work independently and on multiple projects simultaneously, as well as multi-tasking ability is necessary.
Among the key duties of this position are the following:
Works along with other staff, and integrates molecular biological techniques, like PCR, Western blot, Southern/Northern blot, radiolabel DNA probes, to analyze cell line gene editing and animal gene editing projects.
Additionally, provides knowledge of and hands-on ability to perform plasmid construction, viral vector construction, preparation of viral supernatant and infection with viral particles are essential.
Responsible for compliance in all areas of training, work and operation of their lab responsibilities is expected.
Inputs data in a timely manner and reports submitted as needed for each project.
Interacts with PIs and investigator staff to discuss results and work to troubleshoot problems encountered in the projects.
Provides data and information of services as needed to help the director set pricing and determine usage for billing and grant support.
Minimum Education and Experience:
Bachelor's of Science degree in medical related field (Biology, Genetics, Cell Biology, Neurobiology, Immunology) or equivalent.
A minimum of two years experience working in a laboratory setting.
Required Knowledge, Skills, and Abilities:
Requires immediate application of gene editing methods without time for training in cell culture methods.
knowledge and practice of laboratory safety practices, superior organization skills, attention to detail and strict timelines are all required.
Must be able to lift 50 lbs and must be able to participate in the Occupational Health Program for animal workers.
Regular workweek may be other than Monday through Friday, occasionally may include weekends and/or holidays as needed.
Must be able to pass pre-employment physical examination.
Previous extensive, experience in cell culture/transfection of adherent and non-adherent cell lines is required.
Master's in Science in medical related field with 1-2 years hands-on laboratory experience or
Bachelor's of Science in medical related field with 2-3 years hands-on laboratory experience.
Experience in gene editing using CRISPR-Cas9 (and other related methods) and cell culture of adherent and non-adherent cell lines is required.
Proper sterile technique and general laboratory skills; pipetting, solution and stock preparation, formula calculations, plasmid construction and preparation.
Equipment used will be: cell electroporators, pipets, balances, centrifuges, hybridization oven, electrophoresis apparatuses, cell culture hoods and incubators, culture shakers, film developers, spectrophotometers, thermocyclers and other general laboratory equipment.
Various computer programs such Microsoft Office suite, DNA analysis programs, web based scientific analysis tools.
Physical Demands and Work Environment:
On prolonged daily bases must be able to perform the physical demands of the job of being able to lift 50 pounds, push and pull heavy loads of equipment, bend, stoop and reach, and work in general laboratory conditions while wearing safety apparel and personal protective equipment (PPE), up to BSL-2. Can be standing, bending, stooping, or reaching for long periods of time.
Potential exposure to animal dander.
Work with animals may be required.
The wearing of PPE could increase body temperature.
Additionally, the position requires the ability to stand or walk on hard surfaces for most of the work day, including slippery surfaces.
The work environment includes potential exposure to zoonotic diseases, handling of cells lines at BSL-2 levels, the use of hazardous/restricted chemicals and radioisotope.
Posting Number: 19ST3679
Location: College Ave (RU-New Brunswick)
Internal Number: 98239
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.