1. Research: \u2022 Cell Culture: derivation, culture, characterization and differentiation of stem cells, day to day maintenance of cell cultures, maintain cell culture records, order reagents, supplies and media; make reagent solutions, culture media; routine equipment maintenance, laboratory analysis by molecular, histological, and immunocytochemical methods.
\u2022 Provide various embryo and stem cell related techniques including assisting in derivation, propagation and characterization of pluripotent stem cells, expression profiling, directed differentiation in vitro and in vivo, genetic modification of cell lines, studies on mitochondrial biology and epigenetic and genetic signature of pluripotent cells, DNA, RNA and protein sample processing, preparation of media, general sterile cell culture laboratory upkeep, (manual and computerized). Assist in planning of experimental design and data analysis.
\u2022 Expand cell banks and establish novel pluripotent stem cell lines from various sources including SCNT, parthenogenesis, direct reprogramming (iPS cells) and ANT.
\u2022 Optimize protocols for cryopreservation of pluripotent stem cells
\u2022 Perform various molecular techniques such as DNA prep, PCR, Immunocytochemistry, Karyotyping.
\u2022 Animal related research procedures such as mice tissue collection, derivation of mouse embryonic fibroblast cells, teratoma injection and analysis, and assisting in mouse colony maintenance.
2. Data Collection/Data Analysis/Record Keeping: \u2022 Collect scientific data pertinent to the research projects; keep records of information obtained during research (which may include database searches)
\u2022 Maintain records in computerized database for cell lines cryopreserved for future use. Maintain the integrity of databases.
3. Report Preparation: \u2022 Prepare reports containing analytical, descriptive, and evaluative content that will be submitted for publication or for contribution to grant applications, progress reports, presentations at national and international meetings, etc. Conduct research of literature and database analysis.
4. Other duties as required or assigned, to fulfill the mission of this laboratory.
As the state's only health and research university, OHSU brings together patient care, research, education of the next generation of health care providers and scientists and community service to improve the health and well-being of all Oregonians.The university is a conduit for learning for more than 2,800 students, providing training to interns, residents and fellows in postgraduate medical and research. It is the only place in Oregon that grants doctoral degrees in medicine, dentistry and nursing. OHSU cares for more than 251,000 patients each year from around the region and takes a leadership role in contributing to solutions involving the health of the state and the nation, such as health care access and transparency, ethics and workforce shortage. Beyond providing critical health care services, quality education and cutting-edge research, OHSU also is a key economic and social force in the Northwest. With an annual operating budget of $2.06 billion and more than 14,000 employees, OHSU is Portland?s largest employer. Its size contributes to its ability to provide... many services and community support activities not found anywhere else in Oregon. For more information visit http://www.ohsu.edu/xd/about/index.cfm