Job Description: You will be working on a project focused on mapping spatiotemporal resolved protein interactions of G protein coupled receptors using quantitative mass spectrometry. This role is an excellent opportunity to learn a number of valuable, cutting-edge techniques in cell biology and proteomics while working with a dynamic and interdisciplinary research team. The candidate will generate cell lines expressing affinity tagged GPCRs and perform proximity labeling and affinity purifications of proteins and their interactors for mass spectrometry analysis. The candidate will also have the opportunity to develop and implement new protocols for mass spectrometry-based proteomics experiments.
The Krogan Lab (http://kroganlab.ucsf.edu) is a highly dynamic and collaborative laboratory focusing on the molecular networks underpinning infectious disease, carcinogenesis and psychiatric disorders. Our ideal applicant is a problem solver with a positive attitude, excellent organizational and interpersonal skills, and the ability to learn and implement methodology efficiently.
Skills required: • Junior rank: Bachelor’s degree in biological sciences or an appropriate scientific field. • Assistant/Associate Rank: Master’s degree, or Bachelor’s degree and 5-10 years of work experience in biological sciences or an appropriate scientific field. • Molecular biology techniques including molecular cloning, PCR, and western blotting. • Cell/tissue culture experience. • Able to work independently and meet deadlines; recognize and resolve problems while maintaining a high level of organization. • Effective verbal and written communication skills to interface with the team to review and analyze the results. • Microsoft Office (Word, Excel, and/or Powerpoint).
Preferred skills: • Generating new cell lines and characterizing them. • DNA/RNA Blotting • Transfection or viral transduction. • Affinity purifications of proteins. • Preparation for samples for mass spectrometric analysis. • Fluorescence microscopy. • Experience with mass spectrometry data. • Experience with managing large datasets and/or databases. • Experience with statistical data analysis.
UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.
Internal Number: JPF02577
About University of California, San Francisco
The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and high-quality patient care. It is the only UC campus in the 10-campus system dedicated exclusively to the health sciences.