Research - Laboratory/Non-Laboratory, Staff/Administrative
Job Duties & Responsibilities 70% - Research Primary duties and responsibilities will include, but are not limited to, studies of virus-host interactions using HIV-1 as a system model. Projects include analyzing HIV-1 interactions with neutralizing antibodies using viral assays, PCR amplification of HIV-1 genes from samples of infected individuals, and monitoring viral active and latent replication in primary cells. The successful candidate will be responsible for developing independently new applications of next generation sequencing (NGS) technology to evolving projects in the lab, including single cell RNA-seq, developing new methods to analyze libraries of HIV-1 Env mutants at high depth using NGS, and constructing new approaches to study HIV-1 reservoir in samples from people who live with HIV-1. The successful candidate will work in BSL2 and BSL2+/BSL3 environment and build and use viral reporter systems to study the interactions of viruses with the cellular machinery. The research work involves RNA-seq and transcriptomic analysis using state-of-the-art instrument for single cell analysis, next generation sequencing, sort of infected cells using flow cytometry, and different molecular biology techniques to clone or modify genes. The work also involves interaction with other lab member(s), participation in lab meeting, and supervision of undergraduate students.
30% - Data Analysis, Presentations, and Manuscript Preparation Analysis of experimental results. The successful candidate will use different computer programs to analyze next generation sequencing results (big data) and to associate cellular genes with viral infection. Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation. Development of research directions according to the main project aims and the ongoing experiments. Preparation of figures and manuscripts based on the results. Reading literature related to the research projects and developing collaborations with other research groups. Preparation of data for presentations
Required Qualifications (Must be documented on resume/application materials)
BS/BA in biology, microbiology, or a similar field and 4 years of related experience or a Master's degree and 2 years of related experience
Strong critical thinking skills and ability to develop independently new directions in the lab
Experience and demonstrated skills in next generation sequencing technology and analysis
Proficiency in bioinformatic tools and experience with big data analysis of next generation sequencing results
Excellent communication and organization skills with exemplary scientific record keeping
Proficiency in Microsoft Office applications, including Word, Excel, and Power Point
Ability to work independently, solve problems, work under pressure and manage multiple tasks
Experience and skills in molecular biology including cloning, site directed mutagenesis, PCR and analyzing DNA sequencing results
Demonstrated experience in immunological methods such as ELISA, antibody characterization, and western blot.
Experience and ability to analyze scientific data, present the research results and prepare manuscripts for publication.
Enthusiastic to work in new lab studying virus host interactions; proactive, open-minded, creative and willing to learn
Advanced degree in biology, microbiology or related field
Experience working individually and in groups
Ability to carry out research protocols from written or oral instructions from lab director
Experience in work with bacteria, human cells/tissues and viruses, including HIV-1, in a BL2+/BSL3 facility
High proficiency in tissue culture work with different cell lines, isolation of RNA, analysis of gene expression using microarrays or RNA-seq, and isolation of Peripheral Blood Mononuclear Cells (PBMC) from blood
Experience with flow cytometry techniques and multi-parameter analysis
Internal Number: 331542
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.