Job Requisition Number: 27076. The California Institute for Quantitative Biosciences (QB3) is one of four Governor Gray Davis Institutes for Science and Innovation established in 2000 to ensure the future of the California economy by promoting research and innovation. QB3 is a cooperative effort between the state of California, private industry, venture capital, and the University of California campuses at Berkeley, San Francisco, and Santa Cruz.
QB3 harnesses the quantitative sciences of physics and engineering to unify our understanding of biological systems at all levels of complexity, from atoms and molecules to cells, tissues, and entire living organisms. QB3 scientists make discoveries that drive the development of technologies, products, and wholly new industries, ensuring that California remains competitive in the 21st century.
QB3’s goals are to fuel the California bioeconomy; to support research and training in quantitative biosciences; and to translate academic research into products and services that benefit society.
This position will provide essential all-round support to the QB3 Macrolab Core Facility (http://qb3.berkeley.edu/macrolab/), but with a primary focus on DNA cloning. The position will: 1) interact with facility customers, 2) plan customer projects, 3) carry out and document technical lab work, 4) contribute to day-to-day laboratory duties and organization.
Laboratory work will be focused on DNA cloning and other molecular biology work, using a wide range of established protocols, but will also include some protein expression and purification. The applicant must have the organizational and interpersonal skills to interact with customers, and demonstrate sufficient independence to perform routine tasks and established protocols with minimal supervision in order to keep the laboratory running at a high level of productivity.
DNA Cloning/Molecular Biology: •Initiate projects with customers, and with minimal supervision, develop an appropriate cloning scheme for the project. •Provide guidance to customers if necessary to meet their needs. •Define the goals and timelines for the project and keep customers and Director updated with progress reports. •Amplification of DNA using PCR, including in 96-well format. •Construction of plasmids using a range of cloning techniques (Gibson, LIC, Gateway etc.) •Analysis of sequencing data. •Design and implementation of more complicated cloning requests, such as co-expression vectors, polycistronic/polypromoter vectors, large scale and high-throughput plasmid purifications, mutagenesis. •Transformation and manipulation of E. coli •Maintain stocks of competent cells and other molecular biology reagents. •Perform all procedures in accordance with NIH recombinant DNA guidelines.
Protein purification: •Protein expression in E. coli, from small-scale expression testing to large-scale expression for bulk purifications. •Purification of recombinant proteins using defined protocols on automated FPLC chromatography systems. •Keep accurate records and prepare clear concise reports for customers.
Laboratory organization •Assist in ordering and stocking common lab items, and in the general upkeep of the laboratory. •Contribute to lab safety. •Contribute to the development of additional services and the improvement of existing ones.•Bachelor or Master’s degree in biochemistry, molecular biology or related field,and/or relevant experience/training •Knowledge of basic methods of molecular biology and extensive knowledge of multiple cloning techniques: including (but not limited to) Gibson, LIC, Gateway. •Ability to design and troubleshoot complicated cloning reactions •Experience with protein expression and purification from E. coli. •Proficiency in using computers for writing and keeping spreadsheets. •Ability to work independently as well as cooperatively as a team member. •Attention to detail and excellent communication skills.
The University of California was chartered in 1868 and its flagship campus - envisioned as a "City of Learning" - was established at Berkeley, on San Francisco Bay. Today the world's premier public university and a wellspring of innovation, UC Berkeley occupies a 1,232 acre campus with a sylvan 178-acre central core. From this home its academic community makes key contributions to the economic and social well-being of the Bay Area, California, and the nation.