Research - Laboratory/Non-Laboratory, Staff/Administrative
The Research Specialist will work in the Division of Gastroenterology and will interface with members of this and other labs in the Division. The position will utilize a solid foundation in basic laboratory principles and techniques associated with molecular and cell biology to perform the required tasks.
The Research Specialist will work in a laboratory which focuses on the mechanisms of intestinal host-pathogen interactions (enterotoxigenic E. coli) using a human primary cell culture model (enteroids/organoids). The candidate will employ techniques for bacterial and human tissue culture and carry out experiments under the initial supervision of the faculty investigator until proven proficient in performing assays independently. An ideal candidate will be motivated to learn new skills, troubleshoot, and maintain honest and open communication with the faculty investigator.
Duties and Responsibilities:
Participates in planning studies and evaluating/interpreting test results utilizing detailed knowledge of research laboratory procedures and practices involving intestinal host-pathogen interactions. These lab-based duties will include:
Maintain enteroid cell lines in tissue culture environment following established protocols
Bacterial culture (BSL2 microorganisms)
Nucleic acid isolation
Quantitative RT-PCR (qPCR)
Phase-contrast and fluorescence microscopy
Enzyme-linked immunosorbent assay (ELISA)
Compile, analyze, and interpret research data using computer software applications.
Prepare summary of experimental protocols and relevant data to present/discuss with the faculty investigator
Apply previous knowledge to troubleshoot experiment
Keep a thorough and organized laboratory notebook to document experiments.
Maintain an orderly laboratory workspace.
Assist in general maintenance of laboratory equipment, supplies, and safety/regulatory tasks.
Essential Job Functions:
Is an integrated member of the research team. May be involved in establishing laboratory protocols. Will be asked to prepare reports on the analysis.
Exercises professional and scientific judgment and assumes responsibility for decisions, consequences, and results. Engages in regular and continuous decision making having an impact on the success of the laboratory.
Exchanges routine information in an appropriate manner requiring good oral and written communication skills. Has operating knowledge of the personal computer and has the ability to search and retrieve information on the role of extracellular matrix proteins in inflammation and fibrosis from the internet.
Bachelor's degree in biology, chemistry, or related field plus one year of laboratory experience performing relevant techniques. Master's degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.
JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience.
Master's degree in a related field with laboratory and independent research experience desired.
Department name: 10002791-SOM DOM Bay Gastroenterology Work Schedule/Hours: M-F; 8-4:30 Location: 720 Rutland Avenue Personnel area: School of Medicine
The successful candidate(s) for this position will be subject to a pre-employment background check.
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The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
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