Research - Laboratory/Non-Laboratory, Staff/Administrative
UCSD Layoff from Career Appointment: Apply by 04/17/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 04/26/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under minimal supervision, the laboratory technician will perform research experiments involving molecular and physiological analyses of neuroendocrinology in mouse models. The technician will assist lab members on neuroscience and endocrinology experiments in mice. The technician will help manage the research mouse colony, including compliance with ACP and IACUC policies. Duties include: analyze, breed, handle, sample, inject, and euthanize mice & conduct surgical procedures on mice. Execute mouse studies under direction of the supervisor with minimal technical supervision.
Collect blood samples from mice for hormone analyses. Perform molecular biological techniques including RNA/DNA extractions, DNA analysis (genotyping), RT-PCR, qPCR, cloning, running gels, and transcription reactions. Make lab solutions and reagents. Perform histology techniques such as brain and tissue collection, brain tissue cutting onto slides (using a cryostat), and preparation of brain tissue samples for microscopy analysis.
Assist lab personnel in the running of histological assays on brain tissue, such as in situ hybridization and immunocytochemistry. Perform microscopy analyses of brain histology assays. Analyze data and help design future experiments. The position also involves directly training undergraduate researchers, performing general lab maintenance, and helping to manage biohazard waste and radioactivity compliance.
Experience with molecular biology research techniques, such as RT-PCR, qPCR, DNA/RNA extraction, cloning, work with plasmids, and gel electrophoresis.
Demonstrated experience handling, breeding, euthanizing, injecting, and genotyping of laboratory mice.
Demonstrated experience with collecting blood, brain, or other tissue from mice.
Experience using cryostat machine (or related equipment/technique) to cut or prepare rodent tissues, such as brain, pituitary, or gonad tissue. Experience cutting or mounting tissue onto microscope slides.
Proven experience with conducting histological assays on rodent tissues (such as brain tissue), including in situ hybridization, immunohistochemistry, and tissue/cell staining.
Demonstrated experience with preparation of solutions/media, sterile technique, and basic molecular biology techniques.
Excellent interpersonal skills to be effective in communicating and interacting with variety of people within a large laboratory setting and in administrative components within and outside the University.
Ability to take initiative and handle responsibility and tasks in a dependent, reliable, high-quality, and efficient manner.
Demonstrated ability to use microscopy techniques to analyze genes/proteins/fluorescence measures in tissue.
Strong organization and communication skills (verbal and written) in order to interact professionally and effectively with faculty, staff, and students.
Experience with studying (in a research setting) neurobiology/neuroscience or endocrinology or rodent reproduction or rodent physiology.
Employment is subject to a criminal background check.
Must have experience with and be willing to perform experiments involving the handling, dissection, and euthanasia of laboratory mice.
Must work with radioactive isotopes, recombinant DNA, biohazardous research materials, and toxic research chemicals.
Must be able to occasionally vary work schedule to accommodate research needs.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.