Research - Laboratory/Non-Laboratory, Staff/Administrative
Please Note: This position is now open to external applicants as of 3/27/19.
UCSD Layoff from Career Appointment: Apply by 03/25/19 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 03/27/19. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Under supervision, incumbent will work as part of a research team utilizing bioengineering methods to develop improved cell-based therapeutics to treat cancer and inflammatory diseases. Aid in performing a range of established techniques including tissue culture, qPCR, ELISA, western blot, fluorescent and time-lapse microscopy, virus propagation and titration, flow cytometry, cell and genetic engineering, and working with vertebrate preclinical models of clinically relevant diseases. Assists with interpreting experimental data and generation of figures for manuscripts. Additional duties include zebrafish husbandry and maintaining a stock of mutant and reporter zebrafish lines including genotyping, assessing health, and breeding to support experimental approaches.
Graduation from high school or a General Education Diploma and two years of laboratory experience or two years of college including courses in the natural, physical or social sciences and one year of laboratory experience; or an equivalent combination of education and experience; and knowledge and abilities essential to the successful performance of the duties assigned to the position.
General background and knowledge of a variety of recombinant DNA techniques including: ligation, transformation, screening for clones, DNA preparation, enzyme restriction digests, gel electrophoresis, RNA preparation, plasmid construction, isolation and analysis, PCR and sequencing.
Experience with molecular biological techniques to include cell culture, protein expression, etc.
Proficiency in microscopy and microinjection.
Solid computer skills using MS Word, Excel, Powerpoint, email, and variety of web-based programs. Demonstrated ability to create and maintain databases, perform standard computer tasks, learn new programs/applications with minimal direction, and exercise fast and accurate typing skills. Demonstrated commitment to update/maintain technology skills with relevant computer software.
Knowledge of basic lab safety requirements, procedures, and EH&S regulations and methods for handling, inventory, storing, clean-up, and waste disposal for biohazardous materials. Ability to maintain safe and orderly laboratory. Ability to maintain inventories and order supplies for lab.
Excellent interpersonal skills with strong, demonstrated ability to communicate orally and in writing. Possess skills of tact, poise, diplomacy, confidentiality and flexibility. Ability and willingness to work independently and/or in a team framework in conjunction with principles of community and with a diverse population of staff, faculty, industry and others. Ability to effectively contribute to discussions regarding procedures and goals of unit. Ability to lead and/or participate in discussions regarding procedures and goals of unit.
College level course work in molecular, cellular, or developmental biology; or equivalent combination of education and experience.
Experience working with viruses (in particular oncolytic viruses), viral propagation and titration.
Experience with operation of Flow Cytometer (analysis and sorting) and experience with analysis of FACS data using Flow Jo.
Experience with immunology, immune oncology, and culturing immune cells.
Experience with operation of confocal microscope.
Experience collecting, processing, and analyzing genomics data.
Knowledge of UC and Departmental policies and procedures related to purchasing.
Experience working with vertebrate (in particular mice and zebrafish).
Experience with lab management to include ordering supplies and maintaining equipment.
Employment is subject to a criminal background check.
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.