A Research Assistant II positionis available within the Program in Molecular Basis of Genetic Diseases led byDr. Hassane Mchaourab in the Department of Molecular Physiology and Biophysicsat Vanderbilt University. The individual will be a key personnel responsiblefor research conducted. This position requires experience in protein expressionand purification and standard molecular biology procedures, the ability to workin a team and knowledge of basic laboratory procedures and record keeping.
The Research Assistant II willsupport ongoing experiments in molecular biology, cell culture and proteinexpression/purification. Motivated individuals will have their own projects andperform structural biology related research. Additional responsibilities willinclude maintenance of common lab supplies including buffers and competentcells, organizing chemical stocks etc.
The Program in Molecular Basisof Genetic Diseases uses the tools of biochemistry and biophysics to understandhow variations in the DNA of individuals are related to phenotypes. Our overallapproach spans the spectrum from basic biochemistry, structuralcharacterization of the target proteins using X-ray crystallography, electroncryo-microscopy (cryo-EM) and Electron Paramagnetic Resonance (EPR), to thegeneration of model organisms such as Zebrafish. A repertoire of biophysicaland biochemical methods such as analytical ultracentrifugation (AUC), multianglelight scattering (MALS), Isothermal titration calorimetry (ITC), surfacePlasmon resonance (SPR) and crosslinking are integrated with structural biologyto generate detailed mechanistic models of how mutations affect proteinfunction.
Duties and Responsibilities
Manage and maintaininventory of lab reagents and equipment
Train new lab personaland collaborators on equipment usage
Generate constructs ofprotein of interest and carry out site directed mutagenesis
Express proteins ofinterest in bacterial or mammalian cells
Purify proteins andperform quality control by standard biochemical methods
Opportunity to performindependent research
Profile of an Ideal Candidate
Bachelor's degree in arelated field is necessary
Master's degree ispreferred
Experience in molecularbiology and cell culture is strongly preferred
Experience in proteinexpression and purification is strongly preferred
About Vanderbilt Benefits
In addition to offering alively community that encourages learning and celebrates diversity, VanderbiltUniversity offers a competitive, flexible benefits package including health,dental, vision, life, accidental death & dismemberment, disabilityinsurance, paid time off, and a 403(b) retirement plan with employer match.Vanderbilt offers tuition assistance to employees, spouses and dependentchildren. Also, after five years of service, Vanderbilt will pay up to 55% ofthe tuition for up to three dependent children to earn a bachelor's degree.Learn more about Vanderbilt's benefits at VUBenefits Overview.
About Vanderbilt University
Vanderbilt is a renownedprivate institution founded in 1873 and located in the heart of Nashville,Tennessee. The university's students and faculty frequently cite Nashville andthe surrounding area as one of the many perks of being a part of the Vanderbiltcommunity. Vanderbilt University is a place where your expertise will bevalued, your knowledge expanded, and your abilities challenged. It is a placewhere your diversity is sought and celebrated. It is a place where employeesknow they are part of something bigger than themselves, take exceptional pridein their work and never settle for what was good enough yesterday. Vanderbiltwas recently ranked #2 for ‘best quality of life’ and ‘great financial aid,’ #5for ‘best run colleges’ and ‘beautiful campus,’ and #6 for both being in a‘college city that students love’ and having ‘great campus/city relations.’
Commitment to Equity, Diversity and Inclusion
Vanderbilt University iscommitted to achieving the goal of a diverse and inclusive academic communityof faculty, staff, and students. We seek individuals who are committed to thisgoal and our campus values.
Internal Number: 1900381
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.